lipofectamine 2000 tm transfection reagent (Thermo Fisher)
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Thermo Fisher
lipofectamine 2000 tm transfection reagent
Lipofectamine 2000 Tm Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+2000+tm+reagent/lipofectamine+2000/pmc12126588-307-30-35
Average 90 stars, based on 1 article reviews
Lipofectamine 2000 Tm Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+2000+tm+reagent/lipofectamine+2000/pmc12126588-307-30-35
Average 90 stars, based on 1 article reviews
lipofectamine 2000 tm transfection reagent - by Bioz Stars,
2026-10
90/100 stars
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Luciferase:Article Title: LncRNA OSER1-AS1 regulates the inflammation and apoptosis of rheumatoid arthritis fibroblast like synoviocytes via regulating miR-1298-5p/E2F1 axis Article Snippet: In the meantime, TargetScan indicated that E2F1 was a downstream objective for miR-1298-5p [ ]. .. Besides, to evaluate the relationship between miR-1298-5p and OSER1-AS1 or E2F1, the co-transaction of 293 T and RA-FLSs cells was made with pGL3 reporter luciferase vector, which containing the 3’-UTR sequence of E2F1-mut and E2F1-wt or RA-FLSs -wt and RA-FLSs -mut and miR-1298-5p mimics or Article Title: Ulinastatin Reduces Cancer Recurrence after Resection of Hepatic Metastases from Colon Cancer by Inhibiting MMP-9 Activation via the Antifibrinolytic Pathway Article Snippet: After 48 h, the supernatants were collected, and the concentrations of plasmin were measured using ELISA kits (CUSABIO BIOTECH, China) according to the manufacturer's instructions. .. HCT116 colon cancer cells were transfected with recombinant plasmid carrying luciferase gene according to the Sequencing:Article Title: LncRNA OSER1-AS1 regulates the inflammation and apoptosis of rheumatoid arthritis fibroblast like synoviocytes via regulating miR-1298-5p/E2F1 axis Article Snippet: In the meantime, TargetScan indicated that E2F1 was a downstream objective for miR-1298-5p [ ]. .. Besides, to evaluate the relationship between miR-1298-5p and OSER1-AS1 or E2F1, the co-transaction of 293 T and RA-FLSs cells was made with pGL3 reporter luciferase vector, which containing the 3’-UTR sequence of E2F1-mut and E2F1-wt or RA-FLSs -wt and RA-FLSs -mut and miR-1298-5p mimics or Negative Control:Article Title: LncRNA OSER1-AS1 regulates the inflammation and apoptosis of rheumatoid arthritis fibroblast like synoviocytes via regulating miR-1298-5p/E2F1 axis Article Snippet: In the meantime, TargetScan indicated that E2F1 was a downstream objective for miR-1298-5p [ ]. .. Besides, to evaluate the relationship between miR-1298-5p and OSER1-AS1 or E2F1, the co-transaction of 293 T and RA-FLSs cells was made with pGL3 reporter luciferase vector, which containing the 3’-UTR sequence of E2F1-mut and E2F1-wt or RA-FLSs -wt and RA-FLSs -mut and miR-1298-5p mimics or Clone Assay:Article Title: Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma Article Snippet: .. CRNN-expressing clones CRNN-C2, C3 (KYSE30) or CRNN-C1 (KYSE180) were transfected with double-stranded siRNAs (Ambion, Carlsbad, CA) with Transfection:Article Title: Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma Article Snippet: .. CRNN-expressing clones CRNN-C2, C3 (KYSE30) or CRNN-C1 (KYSE180) were transfected with double-stranded siRNAs (Ambion, Carlsbad, CA) with Article Title: Ulinastatin Reduces Cancer Recurrence after Resection of Hepatic Metastases from Colon Cancer by Inhibiting MMP-9 Activation via the Antifibrinolytic Pathway Article Snippet: After 48 h, the supernatants were collected, and the concentrations of plasmin were measured using ELISA kits (CUSABIO BIOTECH, China) according to the manufacturer's instructions. .. HCT116 colon cancer cells were transfected with recombinant plasmid carrying luciferase gene according to the Article Title: Up-regulated CD38 by daphnetin alleviates lipopolysaccharide-induced lung injury via inhibiting MAPK/NF-κB/NLRP3 pathway Article Snippet: .. To evaluate the role of in ammation, MLE-12 cells were treated with different concentrations of LPS (0, 1, 2.5, 5, 10, 20μg/ml) for 24 h. To determine the effect of Daph, MLE-12 cells were treated with LPS for 12 h, and then co-treated with different concentrations of Daph (5, 10, 20 μg/ml) the following 12 h. In addition, MLE-12 cells were transfected with CD38 overexpression plasmid (Wegen Biotech, Zhenjiang, China) using Article Title: LncRNA OSER1-AS1 regulates the inflammation and apoptosis of rheumatoid arthritis fibroblast like synoviocytes via regulating miR-1298-5p/E2F1 axis Article Snippet: .. RA-FLSs and 293 T cells lines were transfected with pcDNA3.1 Vector, pcDNA-OSER1-AS1, NC, miR-1298-5p mimics and sh-E2F1 by using Article Title: Cell Type-Specific Dependency on the PI3K/Akt Signaling Pathway for the Endogenous Epo and VEGF Induction by Baicalein in Neurons versus Astrocytes Article Snippet: Epo and VEGF concentrations in the cell lysate and culture media were measured using the respective Quantikine ELISA Kits (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions, and detected using an ELISA reader at a wavelength of 450 nm. .. Cortical neurons were transfected with siRNAs specific for rat 12/15-LOX or HIF-1α mRNA, or with scrambled RNA produced by Silencer Pre-designed siRNA (Ambion, Austin, TX) using Article Title: Dihydromyricetin Reduced Bcl-2 Expression via p53 in Human Hepatoma HepG2 Cells Article Snippet: .. Cells were transfected at 80% confluence with the p53 siRNA plasmid and Article Title: RNA and FUS act in concert to prevent TDP-43 spatial segregation Article Snippet: .. U2OS cells were transfected with 500 ng (1:1 ratio) of plasmids RBP1-RFP-MBD/RBP2-GFP (FUS-RFP-MBD and TDP-RFP-MBD with HuR-GFP, G3BP-GFP, YB1-GFP, Lin28-GFP, LARP6-GFP, SAM68-GFP, and U2AF-GFP) with Recombinant:Article Title: Ulinastatin Reduces Cancer Recurrence after Resection of Hepatic Metastases from Colon Cancer by Inhibiting MMP-9 Activation via the Antifibrinolytic Pathway Article Snippet: After 48 h, the supernatants were collected, and the concentrations of plasmin were measured using ELISA kits (CUSABIO BIOTECH, China) according to the manufacturer's instructions. .. HCT116 colon cancer cells were transfected with recombinant plasmid carrying luciferase gene according to the Plasmid Preparation:Article Title: Ulinastatin Reduces Cancer Recurrence after Resection of Hepatic Metastases from Colon Cancer by Inhibiting MMP-9 Activation via the Antifibrinolytic Pathway Article Snippet: After 48 h, the supernatants were collected, and the concentrations of plasmin were measured using ELISA kits (CUSABIO BIOTECH, China) according to the manufacturer's instructions. .. HCT116 colon cancer cells were transfected with recombinant plasmid carrying luciferase gene according to the Article Title: Up-regulated CD38 by daphnetin alleviates lipopolysaccharide-induced lung injury via inhibiting MAPK/NF-κB/NLRP3 pathway Article Snippet: .. To evaluate the role of in ammation, MLE-12 cells were treated with different concentrations of LPS (0, 1, 2.5, 5, 10, 20μg/ml) for 24 h. To determine the effect of Daph, MLE-12 cells were treated with LPS for 12 h, and then co-treated with different concentrations of Daph (5, 10, 20 μg/ml) the following 12 h. In addition, MLE-12 cells were transfected with CD38 overexpression plasmid (Wegen Biotech, Zhenjiang, China) using Article Title: Dihydromyricetin Reduced Bcl-2 Expression via p53 in Human Hepatoma HepG2 Cells Article Snippet: .. Cells were transfected at 80% confluence with the p53 siRNA plasmid and Over Expression:Article Title: Up-regulated CD38 by daphnetin alleviates lipopolysaccharide-induced lung injury via inhibiting MAPK/NF-κB/NLRP3 pathway Article Snippet: .. To evaluate the role of in ammation, MLE-12 cells were treated with different concentrations of LPS (0, 1, 2.5, 5, 10, 20μg/ml) for 24 h. To determine the effect of Daph, MLE-12 cells were treated with LPS for 12 h, and then co-treated with different concentrations of Daph (5, 10, 20 μg/ml) the following 12 h. In addition, MLE-12 cells were transfected with CD38 overexpression plasmid (Wegen Biotech, Zhenjiang, China) using Produced:Article Title: Cell Type-Specific Dependency on the PI3K/Akt Signaling Pathway for the Endogenous Epo and VEGF Induction by Baicalein in Neurons versus Astrocytes Article Snippet: Epo and VEGF concentrations in the cell lysate and culture media were measured using the respective Quantikine ELISA Kits (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions, and detected using an ELISA reader at a wavelength of 450 nm. .. Cortical neurons were transfected with siRNAs specific for rat 12/15-LOX or HIF-1α mRNA, or with scrambled RNA produced by Silencer Pre-designed siRNA (Ambion, Austin, TX) using |